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Image Search Results
Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry
Article Title: Substrate Binding Switches the Conformation at the Lynchpin Site in the Substrate-Binding Domain of Human Hsp70 to Enable Allosteric Interdomain Communication
doi: 10.3390/molecules23030528
Figure Lengend Snippet: PRE experiments with SBD(∆CDE)-y with spin labeling at residue 537. ( a ) The spin-labeling position in the solution structure of SBD(∆CDE)-y is shown, in which, S537 was exchanged to cysteine to facilitate covalent labeling with MTSL; ( b ) Representative spectral changes caused by the paramagnetic effect. In the left two panels, 2D 1 H- 15 N HSQC spectra are overlaid for the paramagnetic spin labeled sample (red) and for the sample with a diamagnetic label (black) in the absence of the NR-peptide. In the two panels on the right, the corresponding spectral comparison in the presence of 5.0 mM NR-peptide. The concentration of SBD(∆CDE)-y was adjusted to 0.5 mM.
Article Snippet: The SBD(∆CDE) (S537C/L542Y) mutant was incubated with 10-fold molar excess of (1-oxyl-2,2,5,5-tetramethyl-delta(
Techniques: Labeling, Residue, Comparison, Concentration Assay